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phospho synaptotagmin  (R&D Systems)


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    Structured Review

    R&D Systems phospho synaptotagmin
    Alteration of expression pattern of SNARE-related proteins. Representative western blot (left) and densitometric analysis (right) of proteins in the cytosolic (a) and membrane (b and c) fractions prepared from mouse cerebral cortex ( n = 3 per group). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared with control littermates. P-SYT: phosphorylated <t>synaptotagmin-1.</t>
    Phospho Synaptotagmin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+synaptotagmin/Phospho-Synaptotagmin-1+(S309)+Antibody/pmc05727794-41-28-29
    Average 90 stars, based on 2 article reviews
    phospho synaptotagmin - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice"

    Article Title: Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice

    Journal: Neural Plasticity

    doi: 10.1155/2017/4526417

    Alteration of expression pattern of SNARE-related proteins. Representative western blot (left) and densitometric analysis (right) of proteins in the cytosolic (a) and membrane (b and c) fractions prepared from mouse cerebral cortex ( n = 3 per group). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared with control littermates. P-SYT: phosphorylated synaptotagmin-1.
    Figure Legend Snippet: Alteration of expression pattern of SNARE-related proteins. Representative western blot (left) and densitometric analysis (right) of proteins in the cytosolic (a) and membrane (b and c) fractions prepared from mouse cerebral cortex ( n = 3 per group). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared with control littermates. P-SYT: phosphorylated synaptotagmin-1.

    Techniques Used: Expressing, Western Blot, Membrane, Control

    Related Articles

    Expressing:

    Article Title: Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice
    Article Snippet: Cell cytosolic or membrane protein lysates of mouse brain tissues were prepared using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific, 89842).Cell cytosolic or membrane protein lysates of mouse brain tissues were prepared using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific, 89842).. Then, the lysates were separated by SDS–PAGE and probed with specific antibodies: SNAP-25 (Abcam, ab66066), SNAP-23 (Abcam, ab3340), syntaxin (Santa Cruz, sc-12736), Vamp2 (Abcam, ab6276), Munc-18 (SYSY, 116002), Phospho-Synaptotagmin (R&D Systems, PPS085), β -ACTIN (Abcam, ab6276), TUBULIN (Abcam, ab15246), and Na/K ATPase (Millipore, 05-369).. For quantification, the densitometry measurement of each band was first normalized to that of β -ACTIN, TUBULIN, or Na/K ATPase (used as loading control) and then averaged from at least three independent samples.For quantification, the densitometry measurement of each band was first normalized to that of β -ACTIN, TUBULIN, or Na/K ATPase (used as loading control) and then averaged from at least three independent samples.

    Western Blot:

    Article Title: Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice
    Article Snippet: Cell cytosolic or membrane protein lysates of mouse brain tissues were prepared using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific, 89842).Cell cytosolic or membrane protein lysates of mouse brain tissues were prepared using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific, 89842).. Then, the lysates were separated by SDS–PAGE and probed with specific antibodies: SNAP-25 (Abcam, ab66066), SNAP-23 (Abcam, ab3340), syntaxin (Santa Cruz, sc-12736), Vamp2 (Abcam, ab6276), Munc-18 (SYSY, 116002), Phospho-Synaptotagmin (R&D Systems, PPS085), β -ACTIN (Abcam, ab6276), TUBULIN (Abcam, ab15246), and Na/K ATPase (Millipore, 05-369).. For quantification, the densitometry measurement of each band was first normalized to that of β -ACTIN, TUBULIN, or Na/K ATPase (used as loading control) and then averaged from at least three independent samples.For quantification, the densitometry measurement of each band was first normalized to that of β -ACTIN, TUBULIN, or Na/K ATPase (used as loading control) and then averaged from at least three independent samples.

    Membrane:

    Article Title: Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice
    Article Snippet: Cell cytosolic or membrane protein lysates of mouse brain tissues were prepared using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific, 89842).Cell cytosolic or membrane protein lysates of mouse brain tissues were prepared using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific, 89842).. Then, the lysates were separated by SDS–PAGE and probed with specific antibodies: SNAP-25 (Abcam, ab66066), SNAP-23 (Abcam, ab3340), syntaxin (Santa Cruz, sc-12736), Vamp2 (Abcam, ab6276), Munc-18 (SYSY, 116002), Phospho-Synaptotagmin (R&D Systems, PPS085), β -ACTIN (Abcam, ab6276), TUBULIN (Abcam, ab15246), and Na/K ATPase (Millipore, 05-369).. For quantification, the densitometry measurement of each band was first normalized to that of β -ACTIN, TUBULIN, or Na/K ATPase (used as loading control) and then averaged from at least three independent samples.For quantification, the densitometry measurement of each band was first normalized to that of β -ACTIN, TUBULIN, or Na/K ATPase (used as loading control) and then averaged from at least three independent samples.

    Control:

    Article Title: Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice
    Article Snippet: Cell cytosolic or membrane protein lysates of mouse brain tissues were prepared using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific, 89842).Cell cytosolic or membrane protein lysates of mouse brain tissues were prepared using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Scientific, 89842).. Then, the lysates were separated by SDS–PAGE and probed with specific antibodies: SNAP-25 (Abcam, ab66066), SNAP-23 (Abcam, ab3340), syntaxin (Santa Cruz, sc-12736), Vamp2 (Abcam, ab6276), Munc-18 (SYSY, 116002), Phospho-Synaptotagmin (R&D Systems, PPS085), β -ACTIN (Abcam, ab6276), TUBULIN (Abcam, ab15246), and Na/K ATPase (Millipore, 05-369).. For quantification, the densitometry measurement of each band was first normalized to that of β -ACTIN, TUBULIN, or Na/K ATPase (used as loading control) and then averaged from at least three independent samples.For quantification, the densitometry measurement of each band was first normalized to that of β -ACTIN, TUBULIN, or Na/K ATPase (used as loading control) and then averaged from at least three independent samples.



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    R&D Systems phospho synaptotagmin
    Alteration of expression pattern of SNARE-related proteins. Representative western blot (left) and densitometric analysis (right) of proteins in the cytosolic (a) and membrane (b and c) fractions prepared from mouse cerebral cortex ( n = 3 per group). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared with control littermates. P-SYT: phosphorylated <t>synaptotagmin-1.</t>
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    Image Search Results


    Alteration of expression pattern of SNARE-related proteins. Representative western blot (left) and densitometric analysis (right) of proteins in the cytosolic (a) and membrane (b and c) fractions prepared from mouse cerebral cortex ( n = 3 per group). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared with control littermates. P-SYT: phosphorylated synaptotagmin-1.

    Journal: Neural Plasticity

    Article Title: Brain-Specific SNAP-25 Deletion Leads to Elevated Extracellular Glutamate Level and Schizophrenia-Like Behavior in Mice

    doi: 10.1155/2017/4526417

    Figure Lengend Snippet: Alteration of expression pattern of SNARE-related proteins. Representative western blot (left) and densitometric analysis (right) of proteins in the cytosolic (a) and membrane (b and c) fractions prepared from mouse cerebral cortex ( n = 3 per group). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared with control littermates. P-SYT: phosphorylated synaptotagmin-1.

    Article Snippet: Then, the lysates were separated by SDS–PAGE and probed with specific antibodies: SNAP-25 (Abcam, ab66066), SNAP-23 (Abcam, ab3340), syntaxin (Santa Cruz, sc-12736), Vamp2 (Abcam, ab6276), Munc-18 (SYSY, 116002), Phospho-Synaptotagmin (R&D Systems, PPS085), β -ACTIN (Abcam, ab6276), TUBULIN (Abcam, ab15246), and Na/K ATPase (Millipore, 05-369).

    Techniques: Expressing, Western Blot, Membrane, Control